The principle of the Glen Research gel filtration column, Glen Gel-Pak™, is based on size exclusion chromatography that separates molecules according to the hydrodynamic volume of the molecule in aqueous solutions. In gel filtration, the mobile phase for size exclusion is an aqueous solution and the stationary phase is a porous resin. The pores of the resin are sized such that they allow small molecules to enter the pores, yet exclude larger molecules from the pores. The small molecules, such as salts and hydrolyzed protecting groups, diffuse into the pores of the resin and move slowly through the column. The larger molecules, such as DNA or proteins, are excluded from the pores and move quickly through the column. The end result is that the larger molecules elute first in the column void volume while the small molecules are still flowing through the resin of the column.
Glen Gel-Pak columns are ideal for desalting and reaction clean up. They can be used for removal of the ammonium hydroxide deprotection solution and hydrolyzed protecting groups after deprotection. The columns can also be used for the clean up of NHS-labelling reactions to separate the labelled oligo and unlabelled oligo from the unreacted NHS ester, the hydrolyzed label, and n-hydroxysuccinimide, thereby greatly simplifying the downstream purification steps.
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Cambio成立于1985年,是一家私人控股的英国公司,在为大学、大型制药、生物技术、政府机构、农业组织和环境设施的科研实验室提供优质分子生物学试剂和耗材方面享有盛誉。
我们的产品来源于全球领先制造商网络,我们对我们长期的客户和供应商关系感到非常自豪,这促使Cambio致力于:
不断推出新产品
有竞争力的定价
无应力顺序排列
世界一流的技术支持
为了满足日益增长和多样化的市场需求,我们不断增加先进的分子生物学产品到我们广泛的产品组合中。我们目前的产品范围包括以下研究工具:
适体
农业食品诊断
细胞毒性和致突变性检测试剂盒
微阵列
寡合成
细胞培养污染控制
DNA和RNA测序
分子生物学用酶
染色体画
样品制备
分子克隆
转体
体外翻译
基因表达分析
塑料消耗品
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有趣的事实:
1986年,我们的创始董事彼得·迪恩(Peter Dean)与马丁·埃文斯(Martin Evans,现为教授)合作,开发了第一台使用专利“智能加热块(IHB)”技术进行PCR反应的机器,用于实时温度控制