Cambio/5-Ethynyl-dU-CE Phosphoramidite/10-1554-90,Cambio,,Cambio,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Cambio/5-Ethynyl-dU-CE Phosphoramidite/10-1554-90

产品编号：10-1554-90

市场价：¥0.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>其他>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Cambio

公司：Cambio,Inc.

公司分类：

商品介绍

Description

5-Ethynyl-dU offers convenient click conjugation with an azide to generate a label rigidly attached to one of the oligonucleotide bases. 5-Ethynyl-dU is subject to base-catalyzed hydration during cleavage and deprotection, especially when using a strong base or heat. Hydration of an ethynyl group forms a methyl ketone which subsequently blocks potential click reactions. Mild deprotection conditions are necessary when using 5-Ethynyl-dU-CE Phosphoramidite to prevent this side reaction. TIPS-5-Ethynyl-dU-CE Phosphoramidite, containing a protected alkyne, offers broader compatibility with oligonucleotide synthesis and deprotection. Protecting the 5-ethynyl group with a triisopropylsilyl (TIPS) protecting group prevents acid or base catalyzed hydration during oligonucleotide synthesis and workup. A quick treatment with TBAF removes the TIPS protecting group.

Formula: C₄₁H₄₇N₄O₈P

M.W.: 754.81

F.W.: 314.19

Diluent: Anhydrous Acetonitrile

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.

Deprotection: Use ammonium hydroxide and deprotect at room temperature as required by nucleobases. Compatible with UltraMild deprotection. Not compatible with methylamine deprotection.

Storage: Freezer storage, -10 to -30°C, dry

Stability in Solution: 1-2 days

Please Note: All products of baseclick are patent protected and available in collaboration with baseclick. Baseclick GmbH has filed the following patent applications: 1. WO2006/117161, New labelling strategies for the sensitive detection of analytes. 2. WO2008/952775, Click Chemistry for the production of reporter molecules. Baseclick GmbH holds a worldwide license for the research market of the "Click Chemistry" patent from "The Scripps Research Institute": 3. WO03/101972, Copper-catalysed ligation of azides and acetylenes.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200

品牌介绍

Cambio成立于1985年，是一家私人控股的英国公司，在为大学、大型制药、生物技术、政府机构、农业组织和环境设施的科研实验室提供优质分子生物学试剂和耗材方面享有盛誉。

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