The QuickExtract™ DNA Extraction Solution can be used to rapidly and efficiently extract PCR-ready genomic DNA from almost any sample type using a simple, one-tube protocol that takes only 3-8 minutes, depending on the sample. QuickExtract Solution has been used to extract DNA from samples such as hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells, zebrafish organs and scales, and mouse tail snips. The extracted DNA is suitable for PCR analysis, such as genomic, transgenic, or viral DNA screening in animals, or for genetic or environmental research and screening in humans and other organisms.
The QuickExtract method allows for the inexpensive processing of one to hundreds of samples simultaneously, without centrifugation, spin columns or the use of any toxic organic solvent. The method is also compatible with robotic automation.
IMPORTANT NOTE FOR QUICKEXTRACT USERS:
All end-users should bear in mind that QuickExtract Solutions generate crude extracts for PCR. As such many types of PCR inhibitors contained in the original sample will also be present in the crude extract and it is essential to fully control for false negative results in any downstream assays. Furthermore, it is debatable whether all possible types of PCR inhibition can be controlled for adequately using any previously described controls.
It is important to note that BioSearch Technologies/Lucigen/Epicentre QuickExtract Solutions (QuickExtract DNA and QuickExtract RNA) supplied by Cambio Ltd are not validated for use in diagnostic testing (including for SARS-CoV-2). LGC Biosearch and Cambio Ltd take no responsibility for any results obtained. QuickExtract solutions are authorized for Research Purposes ONLY.
Resources
Rapid RNA extraction protocols for SARS-Cov-2 detection
Cui Lab - Washington University - Rapid QuickExtract method
Feng Zhang Lab, Broad Institute – Rapid QuickExtract method
Latest Poster available
QuickExtract – Rapid and efficient extraction of PCR-ready genomic DNA from plant and seed samples
Figure 1. Procedure for obtaining PCR-ready DNA using QuickExtract™ DNA Extraction Solution. |
Figure 2. FailSafe™ PCR amplifications of genomic DNA extracted from a variety of tissues or cells. Buccal cells were extracted using the BuccalAmp™ DNA Extraction Kit, and all other samples with QuickExtract™ DNA Extraction Solution. PCR was performed using primers to amplify the regions indicated: Lanes 1-3, human β-globin; lane 4, transgenic mouse GAPDH; lane 5, E. coli 16S ribosomal RNA gene; lane 6, transgenic SV40 T antigen. |
Figure 3. Extracted DNA from multiple Zebrafish organs using QuickExtract™ DNA Extraction Solution 1.0. A 1-µl aliquot of a 100-µl extracted sample was used to amplify a single-copy crystallin-like gene. Lane 1, 100-bp ladder; lanes 2-3, fins; lanes 4-5, eyes; lanes 6-7, scales; lane 8, no-DNA control. |
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Cambio成立于1985年,是一家私人控股的英国公司,在为大学、大型制药、生物技术、政府机构、农业组织和环境设施的科研实验室提供优质分子生物学试剂和耗材方面享有盛誉。
我们的产品来源于全球领先制造商网络,我们对我们长期的客户和供应商关系感到非常自豪,这促使Cambio致力于:
不断推出新产品
有竞争力的定价
无应力顺序排列
世界一流的技术支持
为了满足日益增长和多样化的市场需求,我们不断增加先进的分子生物学产品到我们广泛的产品组合中。我们目前的产品范围包括以下研究工具:
适体
农业食品诊断
细胞毒性和致突变性检测试剂盒
微阵列
寡合成
细胞培养污染控制
DNA和RNA测序
分子生物学用酶
染色体画
样品制备
分子克隆
转体
体外翻译
基因表达分析
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有趣的事实:
1986年,我们的创始董事彼得·迪恩(Peter Dean)与马丁·埃文斯(Martin Evans,现为教授)合作,开发了第一台使用专利“智能加热块(IHB)”技术进行PCR反应的机器,用于实时温度控制