Applications
For rapid, dependable optimization of PCR, we encourage you to try the FailSafe™ PCR System to obtain the best PCR results with any template up to ~20 kb. However, if you prefer to use another enzyme, Epicentre also has two MasterAmp™ PCR Optimization Kits that will help you to obtain the best possible PCR results with thermostable enzymes frequently used in PCR, such as Taq, Pfu, Tth, Tfl DNA polymerases. These MasterAmp PCR Optimization Kits contain a series of 12 MasterAmp PCR 2X PreMixes that have been meticulously tested and represent a complete range of PCR conditions frequently encountered. Each MasterAmp 2X PreMix contains all components needed for PCR except for the primers, the template, and the PCR enzyme. First, use the MasterAmp™ PCR Optimization Kit (Cat. No. MO7201) to perform PCR with your template, primers, and each of the 12 MasterAmp PreMixes. Select the PreMix that gives optimal results. Then, use the same MasterAmp PCR PreMix to obtain reliable, consistent PCR usng the same DNA target/primer set. | STEP 1. Find the optimal MasterAmp PCR PreMix. Do PCR with your template, primers and PCR enzyme in the 12 PreMixes. | |
A. A 75%-GC-rich ApoE gene region was amplified in total human genomic DNA using PreMixes A-L. MasterAmp PCR PreMix K produced optimal results in Step 1. | ||
STEP 2. Use the same MasterAmp PCR PreMix to obtain reliable, consistent PCR of the same sequence. | ||
B. Subsequent PCR of the ApoE gene with the same primer pair and MasterAmp™ PreMix K gave consistent results. Subsequent PCR results are consistent and reliable using the optimal PreMix in Step 1. |
MasterAmp PCR Optimization Kits; MasterAmp Taq DNA Polymerase; MasterAmp Tfl DNA Polymerase. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser"s own internal research. No other patent rights (such as 5´ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
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Cambio成立于1985年,是一家私人控股的英国公司,在为大学、大型制药、生物技术、政府机构、农业组织和环境设施的科研实验室提供优质分子生物学试剂和耗材方面享有盛誉。
我们的产品来源于全球领先制造商网络,我们对我们长期的客户和供应商关系感到非常自豪,这促使Cambio致力于:
不断推出新产品
有竞争力的定价
无应力顺序排列
世界一流的技术支持
为了满足日益增长和多样化的市场需求,我们不断增加先进的分子生物学产品到我们广泛的产品组合中。我们目前的产品范围包括以下研究工具:
适体
农业食品诊断
细胞毒性和致突变性检测试剂盒
微阵列
寡合成
细胞培养污染控制
DNA和RNA测序
分子生物学用酶
染色体画
样品制备
分子克隆
转体
体外翻译
基因表达分析
塑料消耗品
无论您是在线购买、电话购买还是传真购买,客户满意都是我们的首要任务。如果你很难找到你要找的东西,请给我们打电话或发邮件。我们承诺快速反应和有竞争力的折扣批量购买。
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有趣的事实:
1986年,我们的创始董事彼得·迪恩(Peter Dean)与马丁·埃文斯(Martin Evans,现为教授)合作,开发了第一台使用专利“智能加热块(IHB)”技术进行PCR反应的机器,用于实时温度控制