Description
miniPCR Genotype to Phenotype – PTC Taster Lab
In the PTC Taster Lab you will examine how small genetic (SNPs) can change our ability to perceive the world around us. You will PCR amplify your own TAS2R38 bitter taste receptor gene and use DNA restriction digest analysis to determine whether you carry a taster (dominant) or non-taster (recessive) variant of the gene.
Description
AN ADVANCED PLACEMENT BIOLOGY NOTABLE LAB.
To taste or not to taste, that is the question. In the PTC Taster Lab you will examine how small genetic variations called single nucleotide polymorphisms (SNPs) can change our ability to perceive the world around us. The human sense of taste is composed of an intricate neurophysiological network, but as we’ll see in this lab, it only takes a few small changes in our DNA to change the way we taste.
Alleles of the TAS2R38 taste receptor gene can confer the phenotypic ability to taste the chemical Phenylthiocarbamide (PTC) and other bitter flavors. You will PCR amplify your own TAS2R38 taste receptor gene and use DNA restriction analysis to determine whether you have taster or non-taster version of the gene. You can determine whether you are homozygous or heterozygous for this trait, and correlate your genotype with your own ability to taste PTC (and foods that contain related chemicals, such as broccoli).
This Learning Lab is aligned with Natio
nal Standards and AP Biology objectives. It utilizes the following molecular genetic techniques:
- DNA extraction from cheek cells
- PCR amplification of human genomic DNA
- Restriction digest analysis of human SNPs
- DNA Gel electrophoresis, staining, and visualization
The miniPCR PTC Taster Lab kit contains reagents for 8 lab groups of 4 students each (32 students):
- X-TractTM DNA extraction buffer
- 2X EZ PCR Master Mix, Load-ReadyTM
- PTC Lab Primer Mix, 2X concentrate
- Restriction enzyme
- 100 bp DNA ladder
Please note:
- These reagents require frozen storage.
- Microtubes, PCR tubes, and electrophoresis reagents sold separately (or select the option above for Lab Companion Kit add-on)
Free miniPCR Learning LabTM downloads:
- PTC Taster Lab Instructor’s Guide
- PTC Taster Lab Student’s Guide
- PTC Lab Classroom Slides
- miniPCR Hardy-Weinberg Calculator
Optional: Lab Companion Kit
Add all the consumables you need for a class of 32 students: microtubes (50), PCR tubes (100), agarose (10 blueGel Tabs x 0.4 g ea.), 20X TBE electrophoresis buffer (30 ml), and GelGreen™ DNA stain, 10,000X (20 µl).
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
Cambio成立于1985年，是一家私人控股的英国公司，在为大学、大型制药、生物技术、政府机构、农业组织和环境设施的科研实验室提供优质分子生物学试剂和耗材方面享有盛誉。
我们的产品来源于全球领先制造商网络，我们对我们长期的客户和供应商关系感到非常自豪，这促使Cambio致力于：
不断推出新产品
有竞争力的定价
无应力顺序排列
世界一流的技术支持
为了满足日益增长和多样化的市场需求，我们不断增加先进的分子生物学产品到我们广泛的产品组合中。我们目前的产品范围包括以下研究工具：
适体
农业食品诊断
细胞毒性和致突变性检测试剂盒
微阵列
寡合成
细胞培养污染控制
DNA和RNA测序
分子生物学用酶
染色体画
样品制备
分子克隆
转体
体外翻译
基因表达分析
塑料消耗品
无论您是在线购买、电话购买还是传真购买，客户满意都是我们的首要任务。如果你很难找到你要找的东西，请给我们打电话或发邮件。我们承诺快速反应和有竞争力的折扣批量购买。
Cambio已通过ISO9001:2008注册（查看证书）
有趣的事实：
1986年，我们的创始董事彼得·迪恩（Peter Dean）与马丁·埃文斯（Martin Evans，现为教授）合作，开发了第一台使用专利“智能加热块（IHB）”技术进行PCR反应的机器，用于实时温度控制
