Description
Description
In this lab students will investigate the genotypic basis of an observable phenotype using Rapid Cycling Brassica rapa (RCBr), also known by the trademark name Wisconsin Fast Plants®
(Note: RCBr seeds necessary to complete the lab may be obtained from Carolina Biological.)
These are not supplied in the kit.
Wild-type plants grow with a distinctive purple stem, best observed in the first few days after germination. This purple color is due to the presence of anthocyanin, a common plant pigment. In some RCBr plants, however, a mutation disrupts the anthocyanin production pathway and leads to green stems with no purple color.
The gene responsible for the purple vs. green color is named Anthocyaninless because the mutant form leads to decreased production of the pigment anthocyanin. Using classical crossing techniques, these alleles can be tracked over generations with predictable results. In this lab, you will test the Anthocyaninless gene from different plants for the presence of the mutation that differentiates the purple vs. green stem alleles.
In this way, students will be able to directly link an organism’s phenotype to its genotype.
- Techniques utilized: DNA extraction from plant tissues, Polymerase Chain Reaction (PCR), DNA gel electrophoresis.
- Time required: can be completed in two 45 min. class periods or a single 2-hour block.
- Reagents needed: ‘miniPCR™ Plant Genetics Lab’ reagents kit (included in this product), electrophoresis reagents (available separately), Rapid Cycling Brassica rapa (RCBr) seeds (available from Carolina Biological)
Suggested skill level: Intended for any student seeking familiarity with PCR, DNA gel electrophoresis, Mendelian inheritance, plant genetics, from middle school to college.
The miniPCR Plant Genetics Lab kit contains reagents for 8 lab groups of up to 4 students each (32 students):
- All molecular reagents needed (DPX DNA extraction buffer, primers, EZ PCR Master Mix containing dNTPs, polymerase, loading dye) and DNA ladder.
- Enough for 8 lab groups of 4 students with each group running four genotyping PCR reactions.
- Reagents are compatible with miniPCR™, blueGel™ or other standard PCR and DNA electrophoresis systems
Please note:
No plants are included in this lab. RCBr seeds necessary to complete the lab may be obtained from Carolina Biological.
- These reagents require frozen storage.
- Microtubes and electrophoresis reagents sold separately.
Free miniPCR Learning Lab™ downloads:
- miniPCR™ Plant Genetics Lab Teachers Guide
- miniPCR™ Plant Genetics Lab Student Guide
- miniPCR™ Plant Genetics Lab Classroom Slides
Contact team@minipcr.com for answers key.
Fast Plants® is a registered trademark of the Wisconsin Alumni Research Foundation.
Optional: Lab Companion Kit
Add all the consumables you need for a class of 32 students: microtubes (50), PCR tubes (100), agarose (10 blueGel Tabs x 0.4 g ea.), 20X TBE electrophoresis buffer (30 ml), and GelGreen™ DNA stain, 10,000X (20 µl). NOTE: This add-on item ships UPS GROUND at no additional charge.
If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200
Cambio成立于1985年，是一家私人控股的英国公司，在为大学、大型制药、生物技术、政府机构、农业组织和环境设施的科研实验室提供优质分子生物学试剂和耗材方面享有盛誉。
我们的产品来源于全球领先制造商网络，我们对我们长期的客户和供应商关系感到非常自豪，这促使Cambio致力于：
不断推出新产品
有竞争力的定价
无应力顺序排列
世界一流的技术支持
为了满足日益增长和多样化的市场需求，我们不断增加先进的分子生物学产品到我们广泛的产品组合中。我们目前的产品范围包括以下研究工具：
适体
农业食品诊断
细胞毒性和致突变性检测试剂盒
微阵列
寡合成
细胞培养污染控制
DNA和RNA测序
分子生物学用酶
染色体画
样品制备
分子克隆
转体
体外翻译
基因表达分析
塑料消耗品
无论您是在线购买、电话购买还是传真购买，客户满意都是我们的首要任务。如果你很难找到你要找的东西，请给我们打电话或发邮件。我们承诺快速反应和有竞争力的折扣批量购买。
Cambio已通过ISO9001:2008注册（查看证书）
有趣的事实：
1986年，我们的创始董事彼得·迪恩（Peter Dean）与马丁·埃文斯（Martin Evans，现为教授）合作，开发了第一台使用专利“智能加热块（IHB）”技术进行PCR反应的机器，用于实时温度控制
