Cell Line Information:

Designations: U-2 OS
Organism: Homo sapiens (human)
Tissue: osteosarcoma; bone
Age: 15 years
Gender: female
Ethnicity: Caucasian
Morphology: epithelial
Growth properties: adherent
AntigenExp: Blood Type A; Rh ; HLA A2, Aw30, B12, Bw35, B40( /-)
Products: osteosacoma derived growth factor (ODGF)
Receptors expressed: IGF-I); IGF-II
Depositors: Hellstrom
Karyotype: Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range.
J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

The Transfection Reagent:

U-2 OS cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting U-2 OS cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U-2 OS cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into U-2 OS cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Animal-free
• Simplest and straightforward protocols
• Compatible with serum
• Suitable for Reverse Transfection 
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on U-2 OS cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for U-2 OS cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this U-2 OS Cell Avalanche Transfection Reagent.   FIG. 2. U-2 OS cells were transfected with GFP vector (pEGFP-N3) by using U-2 OS Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

U-2 OS cell Avalanche™ Transfection Reagent (human osteosarcoma cell) can also be used on the following cells with high transfection efficiencies. Saos-2 Cell Vero Cell 293 Cell 786-O Cell DU 145 Cell 22Rv1 Cell VCaP Cell PC-3 Cell AR42J Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200