Cell Line Information:
Cell Line Name: U251
Patient"s Age: 75
Patient"s Sex: M
Tissue Type: CNS
Histology: Glioblastoma-ud; non epithelial, Pleomorphic/astrocytoid
p53 Status: 0
ECAD Methylation: 11
MDR Function: -19
Institution: Uppsala University, Sweden
Reference: Med Biol. 56:184-193, 1978
Biological source: Brain from human
growth mode: Adherent
karyotype: 2n = 46
receptors: PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991)
Comments: Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (Sigma Catalogue number 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. SNB19 are derived with the same individual with U251.
U251 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:
U251 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting U251 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U251 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in U251 cells
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Free of animal-derived components
• Simplest and straightforward protocols to Increase experimental throughput
• Compatible with serum
• Suitable for Reverse Transfection  
• Compatible with transfection in any plate format
• Great pricing
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on U251 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for U251 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this U251 Cell Avalanche® Transfection Reagent.   FIG. 2. U251 cells were transfected with GFP vector (pEGFP-N3) by using U251 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

U251 Cell Avalanche® Transfection Reagent (human glioblastoma cell) can also be used on the following cells with high transfection efficiencies. SF268 Cell SF295 Cell SNB75 Cell SF539 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell BHK-21 Cell 3197-3 Cell NIH/3T3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem then please contact us or telephone +44 (0)1954 210 200