As the simplified version of our most popular and powerful Avalanche®-Omni Transfection Reagent (Avalanche®-Omni), Avalanche®-Everyday Transfection Reagent (Avalanche®-Everyday) has the basic formulation ofAvalanche®-Omni, thus is also an exceptionally powerful and versatile next-generation DNA and siRNA broad spectrum transfection reagent. Avalanche®-Everyday"s transfection efficiency is very close to that of Avalanche®-Omni, whereas the price of Avalanche®-Everyday is only 1/3 that of Lipofectamine® 2000 (L2K). In addition, Avalanche®-Everyday is able to do 3000 x 24-well transfections/1.5 ml as compared to 750-1000 x 24-well transfections/1.5 ml for L2K.
Features:
Data:
Figure 1. The performance of Avalanche®-Everyday Transfection Reagent (Avalanche®-Everyday) is very close to that of Avalanche®-Omni Transfection Reagent (Avalanche®-Omni). Cell line tested here are: HEK-293 (Human embryonic kidney cell line), U251 (Human glioblastoma cell), LOX-IMVI (Human melanoma cell), hiPS (Human induced pluripotent cell), and Hep G2 (Human hepatocellular carcinoma cell). The cells were transfected with enhanced green fluorescent protein (eGFP) expressing vector by using Avalanche®-Omni, Avalanche®-Everyday, and L2K. Regarding to hiPS cells, after transfection, hiPS cells were fixed with PFA, and stained with OCT-4 antibody (red) and DAPI (Blue). The pictures, which were the overlay of eGFP (green), Oct-4 (red), and DAPI (blue), showed that while all of the hiPS kept their pluripotency after transfection, Avalanche®-Omni and Avalanche®-Everyday showed great eGFP expression. |
Avalanche®-Everyday and Avalanche®-Omni are able to achieved more than 90% knockdown of endogenous gene expression in a variety of cell lines and primary cells. |
Figure 2. Avalanche®-Everyday Transfection Reagent ("Everyday") and Avalanche®-Omni Transfection Reagent ("Omni") achieved more than 90% knockdown of endogenous gene expression. (2A). ROCK1 mRNA levels were quantified using qPCR in Hela cells transfected with Target-specific siRNA duplexes (10nM) for human ROCK1 gene or non-silencing siRNA by using "Omni", "Everyday", and Lipofectamine® 2000 ("L2K"). Data were normalized against the 18S rRNA signal. Control Samples were either mock-transected or untreated. Values are normalized to untreated sample. Data are means¡ÀSD (n=3). (B) Actin stress fibers were stained with FITC-labelled phalloidin on the cells in (A) cultured on fibronectin-coated micropattern. Confocal Microscope revealed that the cells transfected with ROCK1 siRNA showed disrupted stress fiber pattern as compared to the cells transfected with non-silencing siRNA using the same transfection reagent "Omni". The use of "Everyday" showed similar disrupted stress fiber pattern as that of "Omni", while the use of "L2K" only showed slightly disrupted stress fiber pattern. Lipofectamine® is a trademark of Life Technologies Corporation. Avalanche® is a trademark of EZ Biosystems™ LLC |
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Cambio成立于1985年,是一家私人控股的英国公司,在为大学、大型制药、生物技术、政府机构、农业组织和环境设施的科研实验室提供优质分子生物学试剂和耗材方面享有盛誉。
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有竞争力的定价
无应力顺序排列
世界一流的技术支持
为了满足日益增长和多样化的市场需求,我们不断增加先进的分子生物学产品到我们广泛的产品组合中。我们目前的产品范围包括以下研究工具:
适体
农业食品诊断
细胞毒性和致突变性检测试剂盒
微阵列
寡合成
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DNA和RNA测序
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有趣的事实:
1986年,我们的创始董事彼得·迪恩(Peter Dean)与马丁·埃文斯(Martin Evans,现为教授)合作,开发了第一台使用专利“智能加热块(IHB)”技术进行PCR反应的机器,用于实时温度控制