Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective.

Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Prepare working dilution on day of use. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid.Extended Storage after Rehydration: Aliquot and freeze at -70°C or below. Avoid repeated freezing and thawing. Alternatively, add an equal volume of glycerol (ACS grade or better) for a final concentration of 50%, and store at -20°C as a liquid. Expiration date: one year from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substratesDilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Horseradish Peroxidase

Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

This product is for in vitro research use only. It is not a medical device and it is not intended for diagnostic or therapeutic purposes.

Cambio成立于1985年，是一家私人控股的英国公司，在为大学、大型制药、生物技术、政府机构、农业组织和环境设施的科研实验室提供优质分子生物学试剂和耗材方面享有盛誉。

我们的产品来源于全球领先制造商网络，我们对我们长期的客户和供应商关系感到非常自豪，这促使Cambio致力于：

不断推出新产品

有竞争力的定价

无应力顺序排列

世界一流的技术支持

为了满足日益增长和多样化的市场需求，我们不断增加先进的分子生物学产品到我们广泛的产品组合中。我们目前的产品范围包括以下研究工具：

适体

农业食品诊断

细胞毒性和致突变性检测试剂盒

微阵列

寡合成

细胞培养污染控制

DNA和RNA测序

分子生物学用酶

染色体画

样品制备

分子克隆

转体

体外翻译

基因表达分析

塑料消耗品

无论您是在线购买、电话购买还是传真购买，客户满意都是我们的首要任务。如果你很难找到你要找的东西，请给我们打电话或发邮件。我们承诺快速反应和有竞争力的折扣批量购买。

Cambio已通过ISO9001:2008注册（查看证书）

有趣的事实：

1986年，我们的创始董事彼得·迪恩（Peter Dean）与马丁·埃文斯（Martin Evans，现为教授）合作，开发了第一台使用专利“智能加热块（IHB）”技术进行PCR反应的机器，用于实时温度控制